Official websites use .gov
A
.gov website belongs to an official
government organization in the United States.
Secure .gov websites use HTTPS
A lock (
) or https:// means you've safely
connected to the .gov website. Share sensitive
information only on official, secure websites.
Culture medium
| Reagent (MW = molecular weight in g/mol) | Final concentration | Amount |
|---|---|---|
| MEM with Earle’s salts and L-glutamine | N/A | 1 L |
| Glucose (MW = 180.0) | 27.8 mM | 5 g |
| Sodium bicarbonate (NaHCO3, MW = 81.01) | 2.5 mM | 200 mg |
| Holo-Transferrin bovine (MW ∼ 80.000) | 1.25 μM | 100 mg |
| Insulin bovine (MW ∼ 5700) | 4.4 μM | 25 mg |
| Fetal bovine serum | N/A | 50 mL |
| B-27™ supplement | N/A | 10 mL |
| Total | N/A | 1 L |
