Fig. 4.

The effect of Mdivi-1 on α-KG activity in human chondrocytes. (A) RT-qPCR was used to determine the mRNA expression of PINK1, n = 6; (B) Western blot was used to determine protein expression of PINK1 and Parkin, n = 3; (C) ROS levels in human chondrocytes, scale bar = 500 μm, n = 3; (D) Safranin O staining assayed the matrix content in chondrocytes, scale bar = 500 μm, n = 3; (E–I) The mRNA expression of ACAN, COL2A1, MMP13, IL-6, and TNF-α were determined through RT-qPCR, n = 6; (J) The protein expression of MMP13, IL-6, and TNF-α were determined by western blot, n = 3. Values are expressed as means ± S.D. *P < 0.05, **P < 0.01 vs. the corresponding control. (For interpretation of the references to color in this figure legend, the reader is referred to the Web version of this article.)