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. 2023 Mar 20;8:132. doi: 10.1038/s41392-023-01399-3

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© The Author(s) 2023

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 1 — Analytical workflow of small molecule metabolites-based metabolomics. The first stage involves experimental design, followed by election of biological subjects, sample collection, preparation, and metabolite extraction. Next is acquisition and processing of data, then data analysis, and finally, making sense of the data through biomarker discovery, and functional interpretation. The images were obtained using the example data provided by the MetaboAnalyst 5.0 and figures created by BioRender