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. Author manuscript; available in PMC: 2023 May 30.
Published in final edited form as: Sci Transl Med. 2022 Nov 30;14(673):eabq6146. doi: 10.1126/scitranslmed.abq6146

Fig. 1. Establishment and characterization of human normal GEJ organoids.

Fig. 1.

(A) A workflow of organoid generation from human primary endoscopic GEJ biopsies. Biopsies of normal GEJ mucosa were taken by upper endoscopy and then minced and enzymatically dissociated. The cell suspension was mixed with Matrigel to initiate 3D organoid culture in the conditioned medium. (B to D) GEJ organoids were analyzed for structural and growth properties at the indicated time points. 3D organoids were photomicrographed under phase-contrast microscopy (B, top) and collected for H&E staining (B, bottom). Scale bars, 50 μm. Average organoid size (C) and viability (D) were determined at each time point. Data are represented as means ± SD; n = 6 biological replicates. *P < 0.05 versus day 1; #P < 0.05 versus day 14; not significant (ns) versus day 24 by ANOVA.