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. Author manuscript; available in PMC: 2023 Oct 3.
Published in final edited form as: Nat Cell Biol. 2022 Oct 3;24(10):1541–1557. doi: 10.1038/s41556-022-00994-w

Extended Data Fig. 5 ∣. RPL22L1b facilitates GBM growth in low pH conditions.

Extended Data Fig. 5 ∣

a, RT-PCR analysis of RPL22L1 splicing in 001, 011 and 022 GBM spheres that were cultivated for 5 days in normal (pH 7.4) or acidified (pH 6.0) medium. b, Immunofluorescent staining of different areas of GBM tumor tissues from patient 1051 for RPL22L1 (green) and DNA (blue). Yellow and red arrows indicate cells with nuclear and cytoplasmic localization of RPL22L1 respectively. c, Colocalization analysis of RPL22L1 and DAPI staining for the same tumor areas as in ‘b’. Pearson’s R value is indicated. d, Representative immunofluorescent staining of 022 GBM cells overexpressing RPL22L1a, RPL22L1b or an empty vector with antibodies against N-terminal part of RPL22L1. e, FACS analysis of caspase 3/7 activity and SYTOX staining of 157 glioma spheres that were transduced with lentiviruses encoding RPL22L1a, RPL22L1b or an empty vector (control) and cultivated in normal (pH 7.4) or acidified medium (pH 6.4) for 8 days.