Critical Commercial Assays |
Infinity Cholesterol assay kit |
Thermo Scientific |
#TR13421 |
Infinity Triglycerides assay kit |
Thermo Scientific |
#TR22421 |
Mouse Insulin ELISA kit |
Crystal Chem |
#90080 |
Mouse Leptin ELISA kit |
Crystal Chem |
#90030 |
Autokit 3-HB kit |
Wako Diagnostics |
#417-73501 |
Quant-iT™ RNA Assay Kit |
Thermo Scientific |
#Q33140
|
Quant-iT™ DNA Assay Kit |
Thermo Scientific |
#Q33120
|
TruSeq Stranded mRNA kit |
Illumina |
#20020595 |
Deposited Data |
Raw files for RNA sequencing |
This paper |
https://www.ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSE190389
|
TMM normalized counts, statistical analyses for differential and rhythmic gene expression, and liver metabolome counts and statistical analyses |
This paper |
https://data.mendeley.com/datasets/wc5wy48x93/draft?a=8aafd31c-bdf9-4782-bffe-10eccb3dd093
|
Experimental Models: Organisms/Strains |
C57BL/6J |
The Jackson Laboratory |
#000664 |
Software and Algorithms |
Prism 6.0 |
GraphPad Software |
https://www.graphpad.com
|
R, v4.0.3 |
R Development Core Team, 2022 |
https://www.R-project.org/
|
Rstudio, v1.3.1093 |
Rstudio: Integrated Development for R, Boston, MA |
https://www.rstudio.com/
|
FastQC, v0.11.5 |
Babraham Bioinformatics, Cambridge, UK |
https://www.bioinformatics.babraham.ac.uk/projects/fastqc/
|
STAR v2.5.3a |
Dobin et al., 2013 |
https://github.com/alexdobin/STAR
|
HOMER v4.10 |
Heinz et al., 2010 |
http://homer.ucsd.edu/homer/
|
DESeq2 v1.24.0 |
Love, Huber and Anders, 2014 |
https://bioconductor.org/packages/release/bioc/html/DESeq2.html
|
edgeR v3.26.7 |
Robinson, McCarthy and Smyth, 2010; McCarthy, Chen and Smyth, 2012 |
https://bioconductor.org/packages/release/bioc/html/edgeR.html
|
Network Analyst |
Zhou et al., 2019a |
https://www.networkanalyst.ca/NetworkAnalyst/home.xhtml
|
Morpheus |
Broad Institute, Cambridge, MA |
https://software.broadinstitute.org/morpheus
|
uwot |
McInnes, Healy and Melville, 2018 |
https://github.com/jlmelville/uwot
|
ClusterProfiler |
Yu et al., 2012 |
https://guangchuangyu.github.io/software/clusterProfiler/
|
Metacycle |
Wu et al., 2016 |
https://github.com/gangwug/MetaCycle
|
Metascape |
Zhou et al., 2019b |
https://metascape.org/gp/index.html#/main/step1
|
WebGestaltR v0.3.1 |
Liao et al., 2019 |
http://www.webgestalt.org/
|
PSEA |
Zhang et al., 2016 |
https://github.com/ranafi/PSEA
|
g:Profiler |
Raudvere et al., 2019 |
https://biit.cs.ut.ee/gprofiler/gost
|
Metaboanalyst v5.0 |
Pang et al., 2021 |
https://www.metaboanalyst.ca/MetaboAnalyst/faces/home.xhtml
|
Other |
Rodent Diet With 45 kcal% Fat |
Research Diets, Inc. |
D12451 |
Rodent regular chow diet |
LabDiet |
5053 - PicoLab Rodent diet 20 extruded, #3002890–712 |