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. Author manuscript; available in PMC: 2023 Mar 20.
Published in final edited form as: Ann Rheum Dis. 2016 Oct 18;76(4):731–739. doi: 10.1136/annrheumdis-2016-209206

Figure 3. Elevated MIP2 detected in the conditioned media from RA and mouse adipose tissues is associated with higher neutrophil migration via p38 activation.

Figure 3.

A. Conditioned media was generated from RA synovium (n=14) and abdominal fat from 10 week fed HFD mouse (n=6) and the proinflammatory factors were assessed by ELISA. B. Peripheral blood (PB) neutrophil chemotaxis was performed in response to 5 to 20% RA adipose conditioned media, n=3. PB neutrophils was sham depleted or neutralized with anti-IL-6, anti-IL-8, anti-CXCL1 or anti-CXCL5 Abs prior to examining (C) chemotaxis or performing (D) Western blot and densitometric analysis of the bands containing cell lysates that are probed for p38 phosphorylation in response to 10% RA adipose media, n=3-5. E. Neutrophils extracted from mouse bone marrow were either sham depleted or neutralized with anti-MIP2, anti-CXCL1 or anti-CXCL5 Abs prior to performing chemotaxis, n=4 or F. densitometric analysis of the Western blot bands for p38 phoshorylation, in response to 10% mouse adipose media, n=4. In B-F, PBS and fMLP (10nM) were used as negative and positive controls. Values are mean ± SE. * indicates p<0.05.