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. Author manuscript; available in PMC: 2024 Jan 20.
Published in final edited form as: ACS Chem Biol. 2022 Dec 19;18(1):59–69. doi: 10.1021/acschembio.2c00678

Figure 3.

Figure 3.

Phosphorylation of wild-type p50 (A) and modified p50 (containing pTyr60) (B) in the presence of Jurkat cell lysate. The results were analyzed by 15% sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE). The relative intensities were determined using ImageQuant software (version 5.2, Molecular Dynamics) based on the 32P signal. 0 min: no Jurkat cell lysate was added; 0.5–60 min after Jurkat cell lysate addition. (C) Time-dependent phosphorylation of the wild-type and modified p50s in the presence of cell lysate from activated Jurkat cells. The band at 30 or 60 min was defined as 100%.