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. Author manuscript; available in PMC: 2024 Jan 20.
Published in final edited form as: ACS Chem Biol. 2022 Dec 19;18(1):59–69. doi: 10.1021/acschembio.2c00678

Figure 4.

Figure 4.

Time-dependent IL-2 promoter DNA binding of phosphorylated wild-type p50 (A) and modified p50 containing pTyr60 (B) in the presence of Jurkat cell lysate. The results were analyzed using 5% native PAGE. 0 min, no activated Jurkat cell lysate was added; 0.5–60 min, following Jurkat cell lysate addition. (C) Time course of binding of wild-type and modified p50s to IL-2 promoter DNA in the presence of cell lysate from activated Jurkat cells. The band having the greatest intensity for each protein was defined as 100%.