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. Author manuscript; available in PMC: 2024 Jan 20.
Published in final edited form as: ACS Chem Biol. 2022 Dec 19;18(1):59–69. doi: 10.1021/acschembio.2c00678

Figure 5.

Figure 5.

Stability of phosphorylated NF-κB in Jurkat cell lysate at room temperature. (A) IL-2 promoter DNA binding of NF-κB containing wild-type p50 protein in the presence of cell lysate from activated Jurkat cells. The NF-κBs were pretreated with 5 mM GTP at room temperature for 10 min before adding IL-2 promoter DNA. (B) IL-2 DNA binding of NF-κB containing 60p-Tyr p50 in the presence of cell lysate from activated Jurkat cells. (C) IL-2 DNA binding of NF-κB containing 60p-CH2-Tyr p50 in the presence of cell lysate from activated Jurkat cells. (D) Time course of binding of the wild-type and modified phosphorylated NF-κB to IL-2 promoter DNA in the presence of Jurkat cell lysate. The bands at 0 min were defined as 100%.