Figure 2.

Symbioflor 2 induces hBD-2 in intestinal epithelial cells in vitro. (a) Incubation of Caco-2 cells with three genotypes of Symbioflor 2 E. coli strain (G₁, G₂, G₃). Caco-2 cells were incubated for 6 h with the heat-killed test bacteria and two probiotic strains as controls (optical density of 0.3). RNA was isolated, reverse-transcribed into cDNA, and the amount of hBD-2 copies was determined by real-time PCR. (b) Time course of hBD-2 induction by Symbioflor 2. Caco-2 cells were treated with the three genotypes (G₁, G₂, G₃) of Symbioflor 2 E. coli strain for 3, 6, 9, and 12 h. (c) Dose dependence, Caco-2 cells were treated for 6 h with elevating doses of the genotype G₂ of the Symbioflor 2 E. coli strain. The data represent the mean±s.e.m. of three independent experiments performed in duplicates. Unpaired t-tests were used to evaluate the statistical significance for comparisons between the groups. The data represent the means±s.e.m. *P<0.05; **P<0.01.

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