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USP36 does not affect the levels of DGCR8 and Drosha. A, Overexpression of USP36 does not increase the levels of DGCR8 and Drosha. H1299, 293, or U2OS cells were transfected with WT USP36 or its catalytically-inactive C131A (CA) mutant and assayed for protein expression by IB. Knockdown of USP36 does not reduce the levels of DGCR8 and Drosha. H1299 and 293 cells transfected with scr or USP36 siRNA (B) and H1299 cells infected with scr or USP36 shRNA (C) were assayed by IB.
