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. 2023 Mar 20;3(3):459–470. doi: 10.1158/2767-9764.CRC-22-0344

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© 2023 The Authors; Published by the American Association for Cancer Research

This open access article is distributed under the Creative Commons Attribution 4.0 International (CC BY 4.0) license.

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SUMOylation of DGCR8 by USP36 does not affect the microprocessor complex formation. A, Abolishing DGCR8 SUMOylation does not affect its binding to Drosha. 293 cells transfected with WT Flag-DGCR8 or its SUMOylation-defective mutants were subjected to co-IP with anti-Flag antibody followed by IB. 2KR and 3KR indicates the Flag-DGCR8^K259R/K707R and Flag-DGCR8^{K259R/K426R/K707R} mutants, respectively. B, Overexpression of USP36 does not promote the binding of DGCR8 with Drosha. 293 cells transfected with Flag-Drosha in the absence or presence of V5-USP36 were assayed by co-IP using anti-Flag followed by IB. C, Knockdown of USP36 does not reduce the binding of DGCR8 with Drosha. 293 cells transfected with Flag-Drosha in the presence of scr or USP36 siRNA were assayed by co-IP using anti-Flag followed by IB.