FIGURE 8.

The SUMOylation of DGCR8 is critical for cell proliferation. A, Establishment of tet-inducible expression of DGCR8. HeLa-TO-Flag-DGCR8^WT or HeLa-TO-Flag-DGCR8^3KR cell lines were incubated in the absence or presence of 2 μg/mL Dox for 24 hours, followed by IB. B, Induced expression of Flag-DGCR8^3KR suppresses cell growth. Above HeLa-TO-Flag-DGCR8^WT and HeLa-TO-Flag-DGCR8^3KR cells were cultured in the absence or presence of 2 μg/mL Dox for 96 hours followed by MTT assays (B). Shown are the fold changes of absorbance from three independent experiments. Data were presented as mean ± SD. P values shown were calculated by Student t test. ΔP < 0.05, compared with cells culture in the absence of Dox. ***, P < 0.001, compared HeLa-TO-Flag-DGCR8^WT cells with HeLa-TO-Flag-DGCR8^3KR cells cultured in the presence of Dox. C, HeLa-TO-Flag-DGCR8^WT and HeLa-TO-Flag-DGCR8^3KR cells were cultured in the absence or presence of Dox for colony formation assays. Shown is one representative colony formation from three independent experiments.