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π–π stacking assessment by Tyr fluorescence quenching. Normalized fluorescence intensity of PEG-PGTyr polymer and corresponding fluorescence intensities of the mRNA, polymer and micelles. Data are presented as the mean ± S.D. (n = 3). Statistical significance was conducted using a two-tailed Student’s t-test. The difference was considered statistically significant with *p < 0.05.
