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. Author manuscript; available in PMC: 2023 Mar 20.
Published in final edited form as: Cell Rep. 2023 Jan 23;42(1):112021. doi: 10.1016/j.celrep.2023.112021

Figure 5. WNT/β-catenin signaling sustains dynamic cell states in EpiLSCs to maintain the formative pluripotency.

Figure 5.

(A and B) Velocities of ESCs and day 1–3 EpiLCs (A) and C1–C3 EpiLSCs (B).

(C) Cell-cycle score presented in the same scSTALT embedding as in (B) (left). Cell-cycle phase distribution and cell entropies were presented cluster-wise in the pie chart (right).

(D) Pseudotime projection of EpiLCs+CHIR compared with day 1–2 EpiLCs and EpiLSCs.

(E) Spearman correlation matrix of the expression profile of day 1–2 EpiLCs, C1–C3 EpiLSCs, and EpiLCs+CHIR.

(F) CHIR increases the expression potential of the 42 driver genes. The inferred driver genes were expressed in the early phase during E4.5–6.5 trajectory (top), and their unspliced/spliced ratios in EpiLC+CHIR are higher than those in day 2 EpiLCs (bottom).

(G) Velocities of the selected genes in day 1–2 EpiLCs, EpiLCs+CHIR, and C2 EpiLSCs.

(H) ATAC-seq openness of FTW cells, EpiLSCs, day 2 EpiLCs+CHIR, and FS cells in the β-catenin binding sites shown by metagene profile plot (top) and heatmap (bottom). 5 kb flanking regions were included.

See also Figure S5.