Skip to main content
. Author manuscript; available in PMC: 2023 Mar 20.
Published in final edited form as: Methods Mol Biol. 2023;2557:349–364. doi: 10.1007/978-1-0716-2639-9_22

Fig. 1.

Fig. 1

Creation of VPS54-KO and rescue of the VPS54-KO by mVPS54-13myc. WB of hTERT RPE1 cell lysates from wild type (WT), VPS54-KO, and two VPS54-KO clones rescued with mVPS54-13myc (R1 and R2) probed with anti-VPS54 antibodies. β-actin was used as a loading control. VPS54-13myc was expressed under the COG4 promoter and therefore the protein level of the expressed VPS54-13myc was similar to the endogenous VPS54 level