Fig. 3.

GARP-KO alters sorting of lysosomal protein cathepsin D. hTERT RPE1 WT, VPS54-KO, and VPS54-KO rescued (R1 and R2) cells were grown on three 6-cm dishes in DMEM/F12-FBS for 16h. Next, cells werer insed with PBS and placed in chemically defined medium for 36 h. Following the incubation for 36 h, the medium was collected, concentrated using 10 k concentrator, and samples were prepared for SDS-PAGE. WB analysis was done by incubating blots with anti-cathepsin D antibody