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. 2023 Mar 12;24(1):2166763. doi: 10.1080/15384047.2023.2166763

Figure 2.

Quantitative analysis of ATP secretion from dying EC cells after treated with ERAD inhibitors and/or RT.

ERAD inhibitor and RT promote ATP release to extracellular space in EC cells. (a and b) Luminescence was measured after 24 hours exposure to increasing doses of EerI or NMS-873 in KYSE70 cells (0–5000 nM). (c) Luminescence was detected after 24 hours exposure to increasing doses of RT (0, 2, 6, 10, and 20 Gy) ± EerI (5000 nM) or NMS-873 (500 nM) in KYSE70 cells. (d-e) Luminescence was measured after 24 hours exposure to increasing doses of EerI or NMS-873 in KYSE140 cells (0–5000 nM). (f) Luminescence was detected after 24 hours exposure to increasing doses of RT (0, 2, 6, 10, and 20 Gy) ± EerI (5000 nM) or NMS-873 (500 nM) in KYSE140 cells. ATP: adenosine 5’-triphosphate; ERAD: endoplasmic reticulum associated protein degradation; EC: esophageal cancer; RT: radiation therapy. Data are means ± SD (N = 3), n.s, not significant,  P > .05; *P ˂ 0.05; **P ˂ 0.01; ***P ˂ 0.001.