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. 2023 Mar 12;24(1):2166763. doi: 10.1080/15384047.2023.2166763

Figure 5.

Dying EC cells provided several “eat me” signals and were engulfed by immune cells. The “eat me” components were correlated with the prognosis of EC patients.

After treated with ERAD inhibitor and/or RT, phagocytosis assay showing the uptake efficacy of EC cells by DCs; the association between intracellular ICD hallmark gene expression and patient survival in EC were also analyzed. (a and b) Quantification was done for KYSE70 and KYSE140 cells engulfed by DCs after exposure to RT (6 Gy) ± EerI (5000 nM) or NMS-873 (500 nM). (c) Low MT-ATP6 expression (N = 73) is associated with favorable disease-free survival. (d) Low CALR expression (N = 91) is associated with poor disease-free survival. (e) Low HMGB1 expression (N = 46) is associated with favorable disease-free survival. CALR: calreticulin; DCs: dendritic cells; EC: esophageal cancer; ERAD: endoplasmic reticulum associated protein degradation; HMGB1: high mobility group protein B1; ICD: immunogenic cell death; MT-ATP6: Mitochondrially Encoded ATP Synthase Membrane Subunit 6; RT: radiation therapy. Data are means ± SD (N = 3), n.s, not significant, P > .05; *P ˂ 0.05; **P ˂ 0.01; ***P ˂ 0.001.