Figure 2.

CircSCN8A inhibits the aggressive progression of NSCLC in vitro. (a) Transfection efficiency of circSCN8A-overexpressing plasmid in A549 and SK-MES-1 cells was determined by qRT-PCR. (b-d) CCK-8, colony formation, and EdU assays were performed to detect the proliferation ability of circSCN8A-overexpressing cells. (e) Wound healing assays were used to determine the effect of circSCN8A overexpression on cell migration. (f and g) Transwell assays were applied to determine the migration and invasion capability in cells with circSCN8A overexpression. (h) Western blot assays of EMT markers (e-cadherin, n-cadherin, and vimentin) in circSCN8A-overexpressing NSCLC cells. Scale bar: 50 μm. **P <0.01, ***P <0.001.