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. 2022 Dec 8;22(7):758–776. doi: 10.1080/15384101.2022.2154543

Figure 3.

Figure 3.

CircSCN8A induces ferroptosis in NSCLC cells. (a and b) CircSCN8A-overexpressing NSCLC cells were treated with ZVAD-FMK (10 μM), Nec-1 (10 μM), or Fer-1 (1 μM) for 24 h, followed by the determination of cell viability and cell death. (c and d) CircSCN8A-transfected NSCLC cells were treated with erastin (10 μM) or Fer-1 (1 μM) for 24 h, followed by CCK-8 assays of cell viability. (d-g) the levels of Fe2+ (d), ROS (e), MDA (f), and GSH (g) were measured in A549 and SK-MES-1 cells with circSCN8A overexpression. (h) Western blot assays were used to detect the protein expression of SLC7A11 and GPX4 in circSCN8A-overexpressing cells. Scale bar: 50 μm. *P <0.05, **P <0.01, ***P <0.001.