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. 2022 Dec 8;22(7):758–776. doi: 10.1080/15384101.2022.2154543

Figure 4.

Figure 4.

CircSCN8A facilitates ACSL4 expression in NSCLC cells. (a) Venn diagram showed the 364 down-regulated mRnas in NSCLC based on 4 datasets including GSE32863, GSE33532, GSE19188, and GSE85841. (b) the intersection between down-regulated mRnas in NSCLC and ferroptosis drivers from FerrDb database. (c and d) qRT-PCR assays of EPAS1, ALOX5, CDO1, ACSL4, IL6, and ATF3 mRNA expression in A549 and SK-MES-1 cells transfected with si-circSCN8A or circSCN8A. (e and f) Western blot assays of ACSL4 protein level in A549 and SK-MES-1 cells with circSCN8A knockdown or overexpression. (g) ACSL4 mRNA expression in 64 pairs of tumor tissues and adjacent non-cancerous samples. (h) Expression correlation between ACSL4 mRNA and circSCN8A in NSCLC tissues. (i) Kaplan-Meier plotter database presented the correlation between circSCN8A expression and prognosis in NSCLC patients. (j and k) Western blot and IHC assays were applied to assess the expression level of ACSL4 protein in NSCLC tissues and matched normal tissues. Scale bar: 100 μm. *P < 0.05, **P < 0.01, ***P < 0.001.