Figure 5.

CircSCN8A suppresses NSCLC progression and induces ferroptosis by promoting ACSL4 expression. A549 and SK-MES-1 cells were co-transfected with circSCN8A and si-ACSL4. (a and b) CCK-8 and colony formation assays of cell proliferation. (c and d) Transwell assays of cell migration and invasion. (e) DCFH-DA probe was used to detect the ROS level. (f-h) the commercial kits were used to determine the levels of Fe²⁺ (f), MDA (g), and GSH (h). Scale bar: 50 μm. *P <0.05, **P <0.01, ***P <0.001.