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. 2022 Dec 8;22(7):758–776. doi: 10.1080/15384101.2022.2154543

Figure 6.

Figure 6.

CircSCN8A serves as a sponge for miR-1290 to facilitate ACSL4 expression.(a) CircInteractome, circBank, and miRDB online databases were used to predict the potential miRnas that might bind to circSCN8A and ACSL4. (b) qRT-PCR analysis of miR-1290 expression in tumor tissues and adjacent normal tissues from NSCLC patients. (c and d) the correlation between miR-1290 expression and ACSL4 or circSCN8A in NSCLC tissues. (e) the predicted binding sites between miR-1290 and circSCN8A. (f) the putative binding sequences of miR-1290 on ACSL4-3”UTR. (g) the dual luciferase reporter assays were performed in A549 and SK-MES-1 cells after transfection with miR-NC or miR-1290 and wild or mutant circSCN8A reporter. (h) the luciferase activities were determined in A549 and SK-MES-1 cells co-transfected with miR-NC or miR-1290 and wild or mutant ACSL4-3”UTR reporter. (i and j) RNA pull-down assays were performed in A549 and SK-MES-1 cells by using a specific biotin-labeled miR-1290 probe, followed by the qRT-PCR to detect the enrichment of circSCN8A and ACSL4 mRNA. (K) qRT-PCR assays were performed to determine the effects of circSCN8A overexpression or knockdown on miR-1290 expression. (l) the ACSL4 protein expression was measured in A549 and SK-MES-1 cells with miR-1290 overexpression or knockdown. (m) the ACSL4 protein levels were determined in NSCLC cells transfected with miR-1290 or/and circSCN8A. *P <0.05, **P <0.01, ***P <0.001.