Figure 8.

CircSCN8A induces ferroptosis in NSCLC cells by inhibiting miR-1290. (a-f) A549 and SK-MES-1 cells were transfected with vector+mir-NC, circScn8a+mir-NC, or circScn8a+mir-1290. (a) Transfected NSCLC cells were treated with erastin (10 μM) for 24 h, followed by the CCK-8 assay of cell viability. (b-e) the levels of Fe2+ (b), ROS (c), MDA (d), and GSH (e) were measured in transfected cells. (f) Western blot assays of SLC7A11 and GPX4 protein expression in transfected cells. Scale bar: 50 μm. *P < 0.05, **P < 0.01, ***P < 0.001.