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. Author manuscript; available in PMC: 2024 Jan 17.
Published in final edited form as: Anal Chem. 2023 Jan 2;95(2):659–667. doi: 10.1021/acs.analchem.2c02042

Figure 1. Workflow for BAMM preparation method and comparison to published workflows.

Figure 1.

a) To perform the BAMM workflow, n-butanol-based monophasic solvent and magnetic beads are first added to sample (1). After a brief vortex, the sample is incubated on ice for 5 min (2). Unbound metabolites and lipids are then removed (3), and protein is digested for 40 min at 60 °C (4). Metabolites, lipids, and peptides are then prepared for analysis (5–6). Comparison of the estimated number of steps (b) and hours (c) necessary to prepare metabolites, lipids, and proteins with the BAMM method and various published methods.