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a) Number of identified proteins from a mouse brain digest when using beads and performing both the 100% acetonitrile wash and 70% ethanol wash, only the 70% ethanol wash, only the 100% acetonitrile wash, and neither wash. b) Number of identified proteins from mouse brain digested overnight without beads, overnight with beads, and at 60 °C with beads. The beads used were SeraSil-Mag 700 nm beads.
