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. Author manuscript; available in PMC: 2023 Mar 20.
Published in final edited form as: Mol Cell. 2023 Feb 16;83(5):746–758.e5. doi: 10.1016/j.molcel.2023.01.024

KEY RESOURCES TABLE.

REAGENT or RESOURCE SOURCE IDENTIFIER
Bacterial and Virus Strains
BL21-AI competent cells ThermoFisher C607003
DH5a ThermoFisher 18258012
NiCo21(DE3) Competent E. coli NEB C2529H
BL21(DE3) cells NEB C25257H
Chemicals, Peptides, and Recombinant Proteins
Type IC Cas recombinant proteins This study N/A
Anti-CRISPR IF2 recombinant protein This study N/A
Anti-CRISPR IC4 recombinant protein This study N/A
Critical Commercial Assays
Q5 Site-Directed Mutagenesis Kit NEB E0554S
Gibson Assembly Cloning Kit NEB E5510S
Q5 High Fidelity 2X Master MIx NEB M0492S
Q1Aprep Spin Miniprep Kit Qiagen 27104
Deposited Data
Type I-C Cascade Apo Structure This study EMD-27402PDB 8DEX
Type I-C Cascade Partial R-loop Structure This study EMD-27403PDB 8DFA
Type I-C Cascade Full R-loop Structure This study EMD-27939PDB 8DEJ
Type I-C Cascade bound to AcrIF2 This study EMD-27412PDB 8DFS
Type I-C Cascade bound to AcrIC4 This study EMD-27409PDB 8DFO
Type I-C Cascade-Cas3 plasmid used for in vivo assay This study addgene #196400
Mendeley data This study https://doi.org/10.17632/spp853ngyh.1
AcrIC4 plasmid used for type I-C Cascade in vivo assay This study addgene #196403
AcrIF2 plasmid used for type I-C Cascade in vivo assay This study addgene #196402
type I-C Cascade target plasmid used for in vivo assay This study addgene #196399
Oligonucleotides
Primers and DNA substrates Table S3 N/A
Recombinant DNA
Plasmids Table S2 N/A
Software and Algorithms
cryoSPARC Punjani et al.41 https://cryosparc.com/
LEGINON Suloway et al., 200542 https://nramm.nysbc.org/software/
WARP Tegunov et al.43 http://www.warpem.com/warp/
CHIMERAX Petterson et al.44 http://plato.cgl.ucsf.edu/chimerax/download.html
COOT Emsley et al.45 https://www2.mrc-lmb.cam.ac.uk/personal/pemsley/coot/
ISOLDE Croll et al.46 https://isolde.cimr.cam.ac.uk/
PHENIX Adams et al.47 https://phenix-online.org/
SerialEM Mastronarde48 https://bio3d.colorado.edu/SerialEM/
cryoSPARC Live Punjani et al.27 https://cryosparc.com/live
AlphaFold Varadi et al.26 https://github.com/deepmind/alphafold