Skip to main content
. 2023 Jan 30;324(4):R470–R479. doi: 10.1152/ajpregu.00241.2022

Figure 4.

Figure 4.

Effect of adult deletion of all three PGC-1s on skeletal muscle ETC expression and mitochondrial content. PGC-1α, PGC-1β, and PRC mRNA expression (A); nuclear-encoded ETC mRNA expression (B); protein expression of ETC subunits (C); densitometry quantification of ETC subunits protein expression to Ponceau S staining in gastrocnemius muscle (D); time to exhaustion (E); citrate synthase activity (F); mtDNA-to-nDNA (mtDNA/nDNA) ratio (G); VDAC protein expression (H); densitometry quantification of VDAC protein expression normalized to Tuba protein expression in gastrocnemius muscle in adult-inducible skeletal muscle-specific PGC-1α/PGC-1β/PRC knockouts (iMS-PGC-1-TKO) and control littermates (I). Data are presented as means ± SE; n = 4–5 per group; *P < 0.05, **P < 0.01, ***P < 0.001 compared with control as determined by unpaired t test. ETC, electron transport chain; KO, knockout; mtDNA, mitochondrial DNA; nDNA, nuclear DNA; PGC-1, peroxisome proliferator-activated receptor γ coactivator-1; PRC, PGC-1-related coactivator; VDAC, voltage-dependent anion channel.