Figure 6.
Other Cbln family members are not expressed by developing CSN. Prior differential gene expression analysis (Sahni et al., 2021a) identified Cbln1 (inset in I) as expressed by CSNmedial. In that dataset, Cbln2 (A), Cbln3 (E), and Cbln4 (I) exhibit no expression by CSNmedial (blue) or CSNBC-lat (red) at P1, P4, and P7. Chromogenic in situ hybridization at P1 (B, F, J), P4 (C, G, K), and P7 (D, H, L) confirms that Cbln3 and Cbln4 are not expressed in Layer V of the developing neocortex and that Cbln2 is mostly excluded from Layer V. Neurons expressing Cbln2 in Layer V are likely callosal projection neurons (Arlotta et al., 2005). Scale bars are 100 µm for insets and 500 µm for other images.