FIG. 3.

Nhp6 and Cdc68 proteins have related effects. (A) Cdc68 function helps to maintain viability upon nitrogen starvation. Cells of the cdc68-Δ922 strain DE4B-20a and the CDC68 strain DE4B-20b were grown on solid rich medium, replica plated to enriched synthetic medium lacking (NH₄)₂SO₄, incubated 4 days, and then replica plated to rich medium and incubated 2 days to allow growth after starvation. All incubations were carried out at 23°C. (B) Nhp6 facilitates CP function. Cells of the cdc68-Δ922 Δnhp6a/b strain NB4B-3b harboring p314-NV5 or pRS314, the CDC68 Δnhp6a/b strain NB6040 harboring pRS314, and the cdc68-Δ922 NHP6A NHP6B strain DE4B-20a harboring pRS314 were grown on enriched selective medium at 23°C, replica plated to the same medium, and incubated at 30 and 34°C. (C) Cells of the cdc68-Δ922 Δnhp6a/b strain NB4B-1c harboring p314-NV5 or pRS314 were grown on solid enriched selective medium, replica plated to enriched selective medium lacking (NH₄)₂SO₄, incubated 2 days, and then replica plated to rich medium and incubated 6 days to allow growth after starvation.