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Pob3-Nhp6a fusion protein. (A) Schematic of the Pob3-Nhp6a protein tagged with V5 epitope and His6 tract; single-letter amino acid abbreviations are used. (B) The Pob3-Nhp6a-V5-His6 fusion protein is intact in vivo. A whole-cell extract (350 mM NaCl extraction buffer) from Δnhp6a/b POB3-NHP6A cells (strain NB6249) was resolved (6-10-15% gel) and analyzed by Western blotting with anti-V5 antibody.
