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The Pob3-Nhp6a protein provides Pob3 and Nhp6a activity in vivo. Cells of the Δnhp6a/b POB3-NHP6A-V5-His6 strain NB6249 (designated Δa/b POB3-NHP6a), the Δnhp6a/b POB3 strain RJY6249 (designated Δa/b), and the NHP6A NHP6B POB3-V5-His6 strain NB6244 (designated wt) were grown on solid rich glucose medium at 23 and 38°C, on rich galactose medium at 23°C, and on rich glucose medium at 23°C following a 5-day incubation at 23°C on enriched medium lacking (NH4)2SO4 (designated N-starvation).
