FIG. 6.

The Pob3-Nhp6a protein forms a stable complex with Cdc68. (A) Material in the 200,000 × g supernatant fraction from cells of strain NB6249 was size fractionated by gel filtration (4) and analyzed by Western blotting using anti-Cdc68 and anti-V5 antibodies. Size markers (Bio-Rad) were cofractionated with the extract. (B) Equal volumes of the same 200,000 × g supernatant were treated with anti-Cdc68 or anti-V5 antibody and the resultant immunoprecipitates and corresponding supernatants were resolved (8% gel) and analyzed by Western blotting with anti-Cdc68 and anti-V5 antibody.