TABLE 1.
Yeast strains
| Strain | Genotype | Source or reference |
|---|---|---|
| W303-1a | MATaleu2-3,112 ura3-52 his3-11,15 ade2-1 trp1-1 can1-100 | |
| W303-1b | MATα leu2-3,112 ura3-52 his3-11,15 ade2-1 trp1-1 can1-100 | |
| NQ6811 | MATaleu2-3,112 ura3-52 trp1Δ1 his4-912δ lys2-128δ CDC68-200N POB3-200N, expressing HA-Cdc68 and HA-Pob3 proteins | 5 |
| NB6241 | W303-1b with NHP6A-V5-His6 replacing NHP6A | p303-NΔXN transformant |
| RJY6249 | MATaleu2-3,112 ura3-1 his3-11,15 ade2-1 trp1-1 can1-100 adl1 Δnhp6a::URA3 Δnhp6b::LEU2 | 36 |
| NB6040 | RJY6249 with his3::HIS3 | pRS303 transformant |
| BM404 | MATaura3-52 his4-912δ lys2-128δ suc2ΔUAS | F. Winston |
| DE4B-20a | MATα cdc68-Δ922 leu2-3,112 ura3-52 trp1-1 his4-912δ lys2-128δ | DE4B (13) segregant |
| DE4B-20b | MATaleu2-3,112 ura3-52 trp1-1 his4-912δ lys2-128δ | DE4B (13) segregant |
| NB4B-1c | MATα cdc68-Δ922 leu2-3,112 ura3 trp1-1 his4-912δ Δnhp6a::URA3 Δnhp6b::LEU2 | NB6040 × DE4B-20a segregant |
| NB4B-3b | MATα cdc68-Δ922 leu2-3,112 ura3 trp1-1 lys2-128δ Δnhp6a::URA3 Δnhp6b::LEU2 | NB6040 × DE4B-20a segregant |
| AW11-9a | MATα leu2-3,112 ura3-52 trp1-1 his4-912δ lys2-128δ suc2ΔUAS | BM404 × DE4B-20a segregant |
| NB39-11a | MATα leu2-3,112 ura3 trp1-1 ade2-1 his4-912δ lys2-128δ Δnhp6a::URA3 Δnhp6b::LEU2 suc2ΔUAS | NB6040 × AW11-9a segregant |
| NB39-18a | MATaleu2-3,112 ura3 trp1-1 ade2-1 his4-912δ lys2-128δ Δnhp6a::URA3 Δnhp6b::LEU2 | NB6040 × AW11-9a segregant |
| NB39-24b | MATaleu2-3,112 ura3 trp1-1 his4-912δ lys2-128δ Δnhp6a::URA3 Δnhp6b::LEU2 Ade+ | NB6040 × AW11-9a segregant |
| FY300 | MATaleu2Δ1 ura3-52 his4-912δ lys2-128δ spt5-194 Ade+ | 49 |
| FY1671 | MATα leu2Δ1 ura3-52 his4-912δ lys2-128δ Δppr2::hisG | 19 |
| NB2Δp-28b | MATα leu2 ura3-52 trp1-1 his4-912δ lys2-128δ Δppr2::hisG Δnhp6a::URA3 NHP6B Ade+ | (FY1671 × NB39-24a) × NB39-24a segregant |
| NB40 | NB39-18a × AW11-9a | |
| NB40-1aF | MATaleu2-3,112 ura3 trp1-1 his4-912δ lys2-128δ Δnhp6a::URA3 Δnhp6b::LEU2 with POB3-NHP6A-V5-His6 replacing POB3 | NB40 segregant transformed with pPNT |
| FY711 | MATα leu2-3,112 ura3-52 his4-912δ lys2-128δ snf5-5::URA3 | 4 |
| NB6244 | W303-1a with POB3-NHP6A-V5-His6 replacing POB3 | pPNT transformant |
| NB6249 | RJY6249 with POB3-NHP6A-V5-His6 replacing POB3 | pPNT transformant |
| NB6251 | NB6249 with trp1::HIS3 | pTH4 (11) transformant |