Table 1.
Cryo-EM data collection, refinement and validation parameters and statistics
| Data collection | |
|---|---|
| Voltage | 300 |
| Magnification | 130,000 |
| Camera | K3 super resolution |
| Slit width (eV) | 20 |
| Super-resolution pixel size (Å) | 0.3265 |
| Binned pixel size (Å) | 0.653 |
| Dose rate (e/px/s) | 18.5 |
| exposure time (s) | 1.0 |
| total dose (e/Å2) | 43.385 |
| fractions | 43 |
| dose per frame (e/Å2/frame) | 1.009 |
| defocus range (µm) | -0.5 to -2.5 |
| Movies collected | 13,770 |
| Data processing and refinement | |
| Particles | 227,533 |
| Chains | 4 |
| Atoms | 4908 |
| Residues | 634 |
| Bonds (RMSD) | |
| Length (Å) | 0.003 |
| Angles (°) | 0.6 |
| MolProbity score | 2.04 |
| Clash score | 13.98 |
| Ramachandran plot (%) | |
| Outliers | 0 |
| Allowed | 5.75 |
| Favored | 94.25 |
| Rotamer outliers (%) | 0 |
| Cβ outliers (%) | 0 |
| Peptide plane (%) | 0 |
| CaBLAM outliers (%) | 1.46 |
| Box | |
| Lengths (Å) | 72.48, 86.85, 74.44 |
| Angles (°) | 90, 90, 90 |
| Masked dFSC model (0/0.143/0.5) | 3.3/3.5/3.8 |
| Model vs. Data | |
| CC (mask) | 0.80 |
| CC (box) | 0.78 |
| PDB code | 8EY2 |
| EMDB | EMD-28666 |
| EMPIAR | EMPIAR-10810 |