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. 2001 May;21(10):3514–3522. doi: 10.1128/MCB.21.10.3514-3522.2001

FIG. 3.

FIG. 3

Sum1-1, but not Sum1, interacts with Orc5 in the two-hybrid assay. (A) Filter assay for lacZ expression of strain L40 transformed with a plasmid containing lexA-ORC5 (pTT93) and plasmids containing GAD (pGAD424), GAD-SUM1-1 (pRH02), or GAD-SUM1 (pRH01). (B) Western analysis of extracts from strains used in panel A probed with anti-GAD antibody. The significance of the faint upper band in panel B, which is seen only with GAD-Sum1 and GAD-Sum1-1 fusion proteins, is unknown. β-gal, β-galactosidase.