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. 2023 Mar 20;26(4):106457. doi: 10.1016/j.isci.2023.106457
REAGENT or RESOURCE SOURCE IDENTIFIER
Antibodies

Peroxidase AffiniPure F(ab')₂Fragment Goat Anti-Human IgG, Fcγ Fragment Specific Jackson ImmunoResearch Cat# 109-036-098; RRID: AB_2337596
Anti-6x-His clone HIS.H8 Thermo Fisher Scientific Cat#MA1-21315; RRID: AB_557403

Bacterial and Virus Strains

pNL4-3.Luc.R-.E- NIH ARP Cat#3418
SARS-CoV-2.SctΔ19 This paper N/A
pcDNA3.1(+) GeneArt/Thermo Fisher Scientific Cat#810330DE
pVSV-G Clontech Sánchez-Palomino et al.57

Biological Samples

Pre-pandemic Plasma Samples This paper N/A

Chemicals, Peptides, and Recombinant Proteins

Fetal Bovine Serum Thermo Fisher Scientific Cat#10270106
Dulbecco’s Modified Eagle Medium Thermo Fisher Scientific Cat#41966052
Expi293 Expression Medium Thermo Fisher Scientific Cat#A1435102
Opti-MEM I Reduced Serum Medium Thermo Fisher Scientific Cat#31985070
ExpiFectamine 293 Transfection Kit Thermo Fisher Scientific Cat#A14524
Versene Thermo Fisher Scientific Cat#15040033
Puromycin Thermo Fisher Scientific Cat#A1113803
DEAE-Dextran Sigma-Aldrich Cat#D9885-100G
BriteLite Plus Luciferase PerkinElmer Cat#6066769
SARS-CoV-2 (2019-nCoV) Nucleocapsid-His Recombinant Protein Sino Biological Inc Cat# 40588-V08B
MACS BSA Solution Miltenyi Biotec Cat# 130-091-376
Phosphate Buffered Saline Thermo Fisher Scientific Cat# 10010015
Phosphate-Citrate Buffer with Sodium Perborate Merck Life Science SLU Cat# P4922-50CAP
o-Phenylenediamine Dihydrochloride Merck Life Science SLU Cat# P8787-100TAB
H2SO4 Sigma-Aldrich Cat# 258105-1L-PC

Deposited Data

Group Geo Means and Raw Neutralization Titers https://data.mendeley.com/ https://doi.org/10.17632/3572bvtgsb.1

Experimental Models: Cell Lines

Expi293F GnTI- cells Thermo Fisher Scientific Cat#A39240
HEK293T/hACE2 cells Integral Molecular Cat#C-HA101
Software and Algorithms
GraphPad Prism v9.3.1 GraphPad Software https://www.graphpad.com/scientific-software/prism/
R v3.6.3 R Foundation for Statistical Computing https://www.r-project.org/

Other

GeneArt Gene Synthesis Thermo Fisher Scientific N/A