Antibodies |
|
Peroxidase AffiniPure F(ab')₂Fragment Goat Anti-Human IgG, Fcγ Fragment Specific |
Jackson ImmunoResearch |
Cat# 109-036-098; RRID: AB_2337596
|
Anti-6x-His clone HIS.H8 |
Thermo Fisher Scientific |
Cat#MA1-21315; RRID: AB_557403
|
|
Bacterial and Virus Strains |
|
pNL4-3.Luc.R-.E- |
NIH ARP |
Cat#3418 |
SARS-CoV-2.SctΔ19 |
This paper |
N/A |
pcDNA3.1(+) |
GeneArt/Thermo Fisher Scientific |
Cat#810330DE |
pVSV-G |
Clontech |
Sánchez-Palomino et al.57
|
|
Biological Samples |
|
Pre-pandemic Plasma Samples |
This paper |
N/A |
|
Chemicals, Peptides, and Recombinant Proteins |
|
Fetal Bovine Serum |
Thermo Fisher Scientific |
Cat#10270106 |
Dulbecco’s Modified Eagle Medium |
Thermo Fisher Scientific |
Cat#41966052 |
Expi293 Expression Medium |
Thermo Fisher Scientific |
Cat#A1435102 |
Opti-MEM I Reduced Serum Medium |
Thermo Fisher Scientific |
Cat#31985070 |
ExpiFectamine 293 Transfection Kit |
Thermo Fisher Scientific |
Cat#A14524 |
Versene |
Thermo Fisher Scientific |
Cat#15040033 |
Puromycin |
Thermo Fisher Scientific |
Cat#A1113803 |
DEAE-Dextran |
Sigma-Aldrich |
Cat#D9885-100G |
BriteLite Plus Luciferase |
PerkinElmer |
Cat#6066769 |
SARS-CoV-2 (2019-nCoV) Nucleocapsid-His Recombinant Protein |
Sino Biological Inc |
Cat# 40588-V08B |
MACS BSA Solution |
Miltenyi Biotec |
Cat# 130-091-376 |
Phosphate Buffered Saline |
Thermo Fisher Scientific |
Cat# 10010015 |
Phosphate-Citrate Buffer with Sodium Perborate |
Merck Life Science SLU |
Cat# P4922-50CAP |
o-Phenylenediamine Dihydrochloride |
Merck Life Science SLU |
Cat# P8787-100TAB |
H2SO4
|
Sigma-Aldrich |
Cat# 258105-1L-PC |
|
Deposited Data |
|
Group Geo Means and Raw Neutralization Titers |
https://data.mendeley.com/ |
https://doi.org/10.17632/3572bvtgsb.1 |
|
Experimental Models: Cell Lines |
|
Expi293F GnTI- cells |
Thermo Fisher Scientific |
Cat#A39240 |
HEK293T/hACE2 cells |
Integral Molecular |
Cat#C-HA101 |
Software and Algorithms |
GraphPad Prism v9.3.1 |
GraphPad Software |
https://www.graphpad.com/scientific-software/prism/ |
R v3.6.3 |
R Foundation for Statistical Computing |
https://www.r-project.org/ |
|
Other |
|
GeneArt Gene Synthesis |
Thermo Fisher Scientific |
N/A |