Skip to main content
. 2019 May 28;36(12):1923–1934. doi: 10.1089/neu.2018.6101

FIG. 5.

FIG. 5.

Diurnal regulation of genes in liver from uninjured rats, and from sham/spinal cord injury (SCI) rats at 2 days post-injury. (A) Female and male rat livers express clock genes, and clock gene expression varies through the day. Per2, Cry1, Bmal1, and Rev-erbα displayed rhythmic expression in liver. Cry1 expression was differentially regulated by sex; Rev-erbα showed significant interaction between Zeitgeber time (ZT) and sex. (B) SCI disrupts clock gene expression in the liver. Liver from sham rats showed clock gene variation between ZT6 and ZT18. SCI dysregulated expression of several clock genes, including Per2, Cry1, and Rev-erbα. SCI also ablated time-of-day differences in expression of Cry1 and Rev-erbα. (C) SCI reduced expression in the liver of inflammatory genes, including IL-1b, Tnfa, Cd68, and Iba1 (main effect). SCI also significantly reduced IL-1b and Tnfa at ZT6. For Iba1, there was also a significant main effect of sex (females higher). Black ∼ indicates that females or males for that gene show significant rhythm; red + indicates injury difference (sham vs. SCI), p < 0.05; yellow hourglass indicates time difference, p < 0.05; blue gender symbol indicates sex difference, p < 0.05. Symbols at top of each graph indicates significant main effect; symbols above/below data indicate significant interactions. Color image is available online.