Table 1.
Primer Sequences of Assessed RNAs
| Gene | Protein function | Primer sequences |
|---|---|---|
| b-actin | Housekeeping control | F: TTCCTTCCTGGGTATGGAAT |
| R: GAGGAGCAATGATCTTGATC | ||
| Glut2 | Facilitated glucose transport | F: CTGGGTCTGCAATTTTGTCA |
| R: TGTAAACAGGGTGAAGACCA | ||
| G6pt1 | Glucose transport into endoplasmic reticulum; glucose homeostasis | F: GTGGGTCCTGGACACTGACT |
| R: ACCTCAGAACAGTCCGTAA | ||
| Pck1 | Enzyme critical for gluconeogenesis | F: AGGAGGAAGAAAGGTGGCACCAG |
| R: GGCAGAGAAGTCCAGACCATTATGC | ||
| AldoB | Roles in glycolysis and gluconeogenesis | F: TTGCCAATGGGAAGGGTA |
| R: ATCCTCTGTAGGCGGTTTCC | ||
| Per2 | Circadian clock gene – with Cry, represses Bmal & Clock transcription | F: ACAAGCGGCTGCAGTAGTGA |
| R: TTCAAGGTTGCCAGCGTGCT | ||
| Cry1 | Circadian clock gene – with Per, represses Bmal and Clock transcription | F: GTGGTGGCGGAAACTGCTCTC |
| R: ACTCTGTGCGTCCTCTTCCTGA | ||
| Bmal1 | Circadian clock gene – with Clock, activates Per and Cry transcription | F: AAAATGCAAGGGAGGCCCAC |
| R: TCTAACTTCCGGGACATCGC | ||
| Rev-erba (Nr1d1) | Circadian clock gene – represses expression of core clock proteins | F: AGACGCTGTGCGTTTTGGAC |
| R: TGTGGGAACTGAGAGAAGCC | ||
| IL-1b | Pro-inflammatory cytokine; secreted mainly by microglia/macrophages | F: CCTTGTGCAAGTGTCTGAAG |
| R: GGGCTTGGAAGCAATCCTTA | ||
| Tnfa | Pro-inflammatory cytokine; secreted mainly by microglia/macrophages | F: CAAGGAGGAGAAGTTCCCA |
| R: TTGGTGGTTTGCTACGACG | ||
| Cd68 | Inflammation – expressed by microglia/macrophages; activation marker; cell homing and adhesion | F: CAAGCAGCACAGTGGACATTC |
| R: CAAGAGAAGCATGGCCCGAA | ||
| Iba1 (AIF1) | Inflammation – expressed by all microglia/macrophages; increased with activation; binds calcium & actin | F: GGCAATGGAGATATCGATAT |
| R: AGAATCATTCTCAAGATGGC |