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. Author manuscript; available in PMC: 2024 Mar 1.
Published in final edited form as: Curr Protoc. 2023 Mar;3(3):e713. doi: 10.1002/cpz1.713

Table 1.

Troubleshooting Guide for Basic Protocols 1 and 2.

Problem Possible Cause Solution
All/almost all samples have a correlation value close to 1 (Morpheus after generating Similarity Matrix) Features are not normalized Check if the data were normalized, meaning all features must be in a range from 0 – 1
Cells on Google Colab notebook can’t run Notebook was not copied to user’s Google Drive Add a copy of the Notebook to your own Google Drive by clicking on “Copy to Drive”
UserWarning: KMeans is known to have a memory leak on Windows with MKL (Math Kernel Library) when there are less chunks than available threads. You can avoid it by setting the environment variable OMP_NUM_THREADS=2 Memory leak Set the environment variable OMP_NUM_THREADS = x, being x the value specified on your error output. Follow the solution in this thread on stack_over_flow