Figure 1. A unique form of cell death in glucose-starved SLC7A11high cells.
a, b, Cell death measurement in SLC7A11high UMRC6 cells (a) or 786-O cells overexpressing SLC7A11 and transfected with empty vector (EV) (b) cultured in glucose-containing (+ Glc) or glucose-free (− Glc) medium with or without indicated concentrations of deferoxamine (DFO), Ferr-1, Z-VAD, Nec-1, Nec-2, and CQ for 7 hours (UMRC6 cells) or 3 hours (786-O cells). c, d, Cell death measurement in UMRC6 cells (c) or 786-O cells overexpressing SLC7A11 (d) cultured in glucose-free medium with vehicle DMSO, 10 μM Z-VAD, 10 μM Ferr-1, and 1 mM TCEP for indicated times. e, Western blotting showing ACSL4 protein levels in wild-type (WT) control and ACSL4 knockout (KO) (K1, K2) UMRC6 cells. f, Cell death measurement in WT and ACSL4-KO UMRC6 cells treated with vehicle or 1 μM RSL3 for 8 hours. g, Cell death measurement in WT and ACSL4-KO UMRC6 cells cultured in glucose-free medium for indicated times. h, Western blotting showing BAX and BAK protein levels in WT and BAX/BAK double knockout (DKO) (K1/K2) H460 cells. i, Cell viability measurement in WT and BAX/BAK-DKO H460 cells treated with vehicle or 1 μM STS for 16 hours. j, Cell death measurement in WT and BAX/BAK-DKO H460 cells cultured in glucose-free medium for indicated times. k, Relative ATP levels of 786-O cells overexpressing SLC7A11 and transfected with empty vector (EV) cultured in glucose-free medium for indicated times. l, Cell death measurement in WT and SLC7A11-KO UMRC6 cells cultured in indicated medium for 6 hours. All P values were calculated using two-tailed unpaired Student t-test. Data are mean ± s.d., n = 3 independent repeats except (k) (6 independent repeats). ns: not significant (P > 0.05). All Western blotting was repeated at least twice, independently, with similar results.