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. Author manuscript; available in PMC: 2023 Mar 20.
Published in final edited form as: Nat Methods. 2022 Oct 24;19(11):1449–1460. doi: 10.1038/s41592-022-01643-8

Extended Data Fig. 7 ∣. Passaging and expansion of intestinal organoids in OCTOPUS.

Extended Data Fig. 7 ∣

a. Experimental procedure for passaging organoids in OCTOPUS. Subculture and expansion of (b) mouse and (c) human intestinal organoids grown in OCTOPUS and Matrigel drop. For comparison, culture conditions (for example, seeding density, hydrogel volume, media composition) were kept the same between the two systems. After 7 days of culture, organoids at a given passage number (Passage N) were physically dissociated and then transferred to new OCTOPUS devices or Matrigel drops (Passage N+1) at densities of 200 crypts/100 μl and 100 crypts/60 μl for mouse and human intestinal organoids, respectively. White solid lines in the micrographs show the outline of culture chambers in the OCTOPUS device or a sessile drop of Matrigel. Scale bars, 1 mm.