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. Author manuscript; available in PMC: 2023 Mar 20.
Published in final edited form as: Nat Methods. 2022 Oct 24;19(11):1449–1460. doi: 10.1038/s41592-022-01643-8

Extended Data Fig. 8 ∣. IBD enteroids cultured in Matrigel drops.

Extended Data Fig. 8 ∣

a. When cultured in Matrigel drops, IBD enteroids show properly polarized epithelial cells (top right) that resemble those in the epithelium of normal enteroids. In comparison to IBD enteroids in OCTOPUS, they also retain the structural integrity of the epithelium as visualized by ZO-1 expression (bottom right). Scale bars, 5 μm. b. UMAP plots showing the expression of two representative LINC genes by IBD and normal enteroids cultured in Matrigel drops. c. UMAP plots showing the expression of 4 representative IBD-associated genes by IBD and normal enteroids cultured in Matrigel drops.