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. Author manuscript; available in PMC: 2023 Mar 20.
Published in final edited form as: Nat Methods. 2022 Oct 24;19(11):1449–1460. doi: 10.1038/s41592-022-01643-8

Extended Data Fig. 2 ∣. Diffusion of soluble factors in OCTOPUS.

Extended Data Fig. 2 ∣

a, b. Visualization of 70 kDa FITC-dextran diffusion into the inner and outer regions of the hydrogel scaffolds in Matrigel drop (a) and OCTOPUS (b). The organoids in the inner and outer regions were located 600 μm (OCTOPUS)/2400 μm (Matrigel drop) and 400 μm (both groups) from the hydrogel surface, respectively. Scale bars, 100 μm. c. Temporal profiles of mean fluorescence intensity (MFI) due to dextran diffusion. d, e. Visualization of temporal changes in oxygen concentration in the inner and outer regions of Matrigel scaffolds in conventional drop culture (d) and OCTOPUS (e). Quenching of blue fluorescence shown in the micrographs was caused by an increase in the level of oxygen. f. Temporal profiles of normalized fluorescence intensity due to oxygen diffusion into Matrigel (n=3 independent experiments).