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. Author manuscript; available in PMC: 2023 Mar 20.
Published in final edited form as: Nature. 2022 May 25;606(7914):557–564. doi: 10.1038/s41586-022-04739-5

Extended Data Fig. 3. Identification of TR proteins in reactive astrocytes by immunohistochemistry.

Extended Data Fig. 3.

For immunohistochemical (IHC) detection of TR protein we focused on previously unpublished, TREA-predicted astrocyte reactivity TRs for which sensitive antibodies were commercially available whose specificity was supported by western blots. a. Newly identified TRs co-localized by IHC in reactive astrocytes in thoracic spinal cord (T9-T-10) after LPS treatment. TRs are show in alphabetical sequence. b. Summary of all (newly identified and previously published) astrocyte reactivity TRs after LPS treatment identified here by at least two experimental procedures, either prediction from DEGs by TREA, or prediction based on significant change in motif access determined by ATACseq or by IHC or by all three. c. Newly identified TRs co-localized by IHC in reactive astrocytes in thoracic spinal cord (T9-T-10) after SCI (continued in Extended data figures 4,5). TRs are show in alphabetical sequence. Each immunohistochemical evaluation was repeated at least three times with similar results.