FIG. 8.
Expression of dC40 does not inhibit transformation by the TEL-JAK2 fusion variants. Ba/F3 cells expressing indicated fusion proteins were maintained in the absence of IL-3. Cells (105) were transduced with the MSCV-puro vector containing the murine dC40 sequence or empty vector. After 48 h, cells were selected for puromycin resistance. Cells were counted 4 days postselection. Puromycin-resistant cells were obtained with all Ba/F3 cell lines transduced with MSCV-puro (open bars) and MSCV–puro–dC40 (solid bars). In particular, in comparison with Fig. 2, puromycin-resistant cells were obtained for all three of the TEL-JAK2 fusion variants. Similar results were obtained in replicate experiments.