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Nucleotide incorporation by hPolη opposite the 3′ T residue in a nondamaged template or a (6-4) TT photoproduct-containing template. (A) Incorporation of nucleotides opposite the nondamaged T residue; (B) incorporation opposite the equivalent 3′ T residue of a (6-4) TT photoproduct. A portion of each primer:template substrate is shown at the top. hPolη (0.5 nM) was incubated with DNA substrate (10 nM) and the indicated concentrations of dNTPs for 5 min at 30°C.
