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Nucleotide incorporation opposite the 5′ T of the (6-4) TT photoproduct by yPolζ. (A) Nucleotide incorporation following a G residue opposite the 3′ T of the (6-4) photoproduct; (B) nucleotide incorporation following an A residue opposite the 3′ T of a (6-4) photoproduct. yPolζ (5 nM) was incubated for 3 min at 30°C with the primer:template DNA substrate (20 nM) and with the indicated concentrations of dNTPs.
